Because the thick spore coat is an impediment to analysis of cellular structure, edwards & roux examined the effects of gravity on protoplasts (isolated from ceratopteris gametophytes) and found them to mimic the effects of gravity on the migration of the nucleus and the direction of rhizoid growth in intact spores. Read this essay on ferns come browse our large digital warehouse of free sample essays get the knowledge you need in order to pass your classes and more only at termpaperwarehousecom. Abscisic acid normally inhibits growth and male sexual differentiation (antheridia formation) in gametophytes of the fern ceratopteris abscisic acid resistant mutants show increased growth and sexual differentiation in comparison with the wild type when cultured in the presence of abscisic acid. Ferns are the only major lineage of vascular plants not represented by a sequenced nuclear genome this lack of genome sequence information significantly impedes our ability to understand and reconstruct genome evolution not only in ferns, but across all land plants azolla and ceratopteris are.
Spores of the arsenic hyperaccumulating fern pteris vittata and tetraploid 'c-fern express' (ceratopteris thalictroides) were stably transformed by agrobacterium tumefaciens with constructs containing the p vittata actin promoter driving a gusplus reporter gene reporter gene expression assays were performed on multiple tissues and growth. The polarity of germinating single-celled spores of the fern ceratopteris richardii brogn is influenced by gravity during a time period prior to the first cellular division designated a polarity-determination window after this window closes, control of polarity is seen in the downward (with. Spores were cultured either in fern medium (fm) lacking ceratopteris antheridiogen (a ce) or in conditioned fern medium (cfm) containing exogenous a ce, prepared according to banks cfm is a crude aqueous extract of medium that has supported the growth of wild-type gametophytes for 14 days.
Stable genetic transformation of ferns has proved challenging in the past, and as such our understanding of fern development lags significantly behind almost all other land plant lineages despite adoption of the fern species ceratopteris richardii for laboratory use. Research in my lab focuses on plant evolutionary biology, spanning population genetics to deep phylogeny my main interest is in ferns (including pteridium and ceratopteris), but students in the lab are also working on flowering plants, including eriogonum, populus, allenrolfea, betula, and boechera. The fern ceratopteris richardii is an important model for studies of sex determination and gamete differentiation in homosporous plants plants and growth.
Qualitative analysis of free amino acids of some pteridophytes with special reference to their (ferns and lycophytes) has found largely in ceratopteris. Below is an essay on fern spore germination and the effects of ammonium nitrate from anti essays, your source for research papers, essays, and term paper examples fern spore germination and the effects of ammonium nitrate. Sex-determining genes in the homosporous fern ceratopteris jo ann banks 0 0 department of botany and plant pathology, lilly hall, purdue university , west lafayette, in 47907 , usa haploid ceratopteris gametophytes are either hermaphroditic or male. Genes involved in asexual sporophyte development in i induced apogamy experimentally from the semiaquatic fern - ceratopteris sporophyte vegetative growth and.
View lab report - (sample report) lab-5-report-growth-and-analysis-of-the-ceratopteris-c-fern-life-cycle-observing-f from bio 193 at depaul university growth and analysis of the. Subsequent genetic analysis has shown the ceratopteris species to be clearly allied with those in the genus acrostichum, and ceratopteridaceae is the family name for the clade that is now known to include these two genera some authorities place these two genera within the pteridaceae family. Sodium/potassium selectivity and pleiotropy in we have used the fern ceratopteris richardii to identify and characterize single for growth analysis,. Sex-determining genes in the homosporous fern ceratopteris plants and growth conditions cies sufficiently high for genetic analysis one month after leaves are. Here, we report a transgenic analysis in the fern ceratopteris richardii that reveals a role for leafy in maintaining cell divisions in the apical stem cells of both haploid and diploid phases of the lifecycle.
Ceratopteris richardii is an ideal choice to be among the first ferns sequenced because of its extensive use as a genetic model system and in education (where it is known as c-fern and is grown in k-12 classrooms around the world. Single-celled spores of the fern ceratopteris richardii undergo gravity-directed cell polarity development that is driven by polar calcium currents here we present results that establish a role for nitric oxide (no)/cgmp signaling in transducing the stimulus of gravity to directed polarization of. Ceratopteris richardii: a productive model for revealing secrets of ceratopteris richardii is an aquatic fern grown in another aid in genetic analysis of.
Abstract this study examined whether gravity influences the growth direction of dark-grown gametophytes of the fern ceratopteris richardii analyses of directional growth of gametophytes in response to gravitropic stimulation demonstrated that gametophytes showed negative gravitropism. Search for more papers by this author and ceratopteris richardii (c‐fern), have reported the profile and analysis of gene expression changes,. Generating autotetraploid sporophytes and their use in analyzing mutations affecting gametophyte development in the fern ceratopteris brody analysis in a haploid.
Download citation on researchgate | single gene mutants tolerant to nacl in the fern ceratopteris: characterization and genetic analysis | nacl-tolerant mutants of the fern ceratopteris richardii. Ceratopteris thalictroides watersprite a fern all the leaves emerge from the base and unfurl upwards thinned out even hes amazed out the tremendous growth. In the domain eukarya, the genus that was examined in this lab was ceratopteris, which is also called c-fern this plant is used in research to see how plant growth is affected by various changes that affect plant growth. The cell evaluated is the spore of the fern ceratopteris richardii, which exhibits both perception and response to gravity cdna microarray and q rt-pcr analysis of spores germinating in microgravity onboard nasa space shuttle flight sts-93 revealed changes in the mrna expression of roughly 5% of genes analyzed.